Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 28, Problem 1CAE
Suppose you are culturing a microorganism that produces enough lactic acid to kill itself in a few days.
- a. How can the use of a bioreactor help you maintain the culture for weeks or months? The graph below shows conditions in the bioreactor:
- b. If your desired product is a secondary metabolite, when can you begin collecting it?
- c. If your desired product is the cells themselves and you want to maintain a continuous culture, when can you begin harvesting?
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media during log phase. If a biotechnologist wishes to obtain a secondary metabolite, they will harvest the organism/media during stationary phase. Can you explain this distinction regarding the metabolism of the cell at these two different batch culture growth phases?
3b)A plant suspension culture was used to study the effect of three types of media on the growth of the culture using batch culture approach. Figure 3.1 shows the growth of the plant suspension cultures using Murashige and Skoog (MS), Gamborg B5 and Vacin and Went media. Comment and conclude on the results obtained. Suggest which is the most suitable medium to maintain the suspension cultures with justification.
Chapter 28 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 28 - What is industrial microbiology? Why is it...Ch. 28 - How does commercial sterilization differ from...Ch. 28 - Why is a can of blackberries preserved by...Ch. 28 - Outline the steps in the production of cheese, and...Ch. 28 - Beer is made with water, malt, and yeast; hops are...Ch. 28 - Why is a bioreactor better than a large flask for...Ch. 28 - The manufacture of paper includes the use of...Ch. 28 - Describe an example of bioconversion. What...Ch. 28 - Prob. 9RCh. 28 - NAME IT Van Leeuwenhoek was the first to see this...
Ch. 28 - Foods packed in plastic for microwaving are a....Ch. 28 - Acetobacter is necessary for only one of the steps...Ch. 28 - Use the following choices to answer questions 35:...Ch. 28 - The spoilage of canned foods caused by Geobacillus...Ch. 28 - A heat-resistant fungus that causes spoilage in...Ch. 28 - The term 12D treatment refers to a. heat treatment...Ch. 28 - Which one of the following is not a fuel produced...Ch. 28 - Which type of radiation is used to preserve foods?...Ch. 28 - Which of the following reactions is undesirable in...Ch. 28 - Which of the following reactions is an oxidation...Ch. 28 - Which bacteria seem to be most frequently used in...Ch. 28 - Methylophilus methylotrophus can convert methane...Ch. 28 - Faded worn-look denim is produced with cellulase....Ch. 28 - Suppose you are culturing a microorganism that...Ch. 28 - Researchers at the CDC inoculated apple cider with...Ch. 28 - The antibiotic efrotomycin is produced by...
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