Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 27.2, Problem 3TYK
Summary Introduction

Introduction:

Fish that have lobed fins are called ‘lobed-fins fish’. Lobed-fin fishes are also termed as  Sarcopterygii  or sometimes  Crossopterygii. A lobed-fin fish is a clade that contains tetrapods, coelacanths, lungfishes, etc. The characteristics feature of lobed-fin fishes are pectoral and pelvic (paired) fins that are articulated through the same bone with the pectoral and pelvic girdle.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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