Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
10th Edition
ISBN: 9780133922851
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 27.2, Problem 1CC
Although rare on a per gene basis, new mutations can add considerable
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Describe two main reasons why the proteomes of eukaryotes are usuallymuch larger than their genomes.
In eukaryotes, sexual reproduction is the main process by which new gene combinations are generated. How do bacteria generate new gene combinations in the absence of sexual reproduction?
Nine rII− mutants of bacteriophage T4 were used inpairwise infections of E. coli K(λ) hosts. Six of themutations in these phages are point mutations; theother three are deletions. The ability of the doubly infected cells to produce progeny phages in large numbers is scored in the following chart.1 2 3 4 5 6 7 8 91 − − + + − − − + +2 − + + − − − + +3 − − + − + − −4 − + − + − −5 − − − + +6 − − − −7 − + +8 − −9 −The same nine mutants were then used in pairwise infections of E. coli B hosts. The production of progenyphages that can subsequently lyse E. coli K(λ) hosts isnow scored. In the table, 0 means the progeny do notproduce any plaques on E. coli K(λ) cells; − meansthat only a very few progeny phages produce plaques;and + means that many progeny produce plaques(more than 10 times as many as in the − cases).1 2 3 4 5 6 7 8 91 − + + + + − − + +2 − + + + + − + +3 0 − + 0 + + −4 − + − + + +5 − + − + +6 0 0 − +7 0 + +8 − +9 −a. Which of the mutants are the three deletions? Whatcriteria did…
Chapter 27 Solutions
Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
Ch. 27.1 - Prob. 1CCCh. 27.1 - Contrast the cellular and DNA structures of...Ch. 27.1 - Prob. 3CCCh. 27.2 - Although rare on a per gene basis, new mutations...Ch. 27.2 - Distinguish between the three mechanisms by which...Ch. 27.2 - In a rapidly changing environment, which bacterial...Ch. 27.2 - WHAT IF? If a nonpathogenic bacterium were to...Ch. 27.3 - Distinguish between the four major modes of...Ch. 27.3 - A bacterium requires only the amino acid...Ch. 27.3 - WHAT IF? Describe what you might eat for a...
Ch. 27.4 - Explain how molecular systematics and metagenomics...Ch. 27.4 - WHAT IF What would the discovery of a bacterial...Ch. 27.5 - Explain how prokaryotes, though small, can be...Ch. 27.5 - MAKE CONNECTIONS Review Figure 10.6. Then...Ch. 27.6 - Identify at least two ways that prokaryotes have...Ch. 27.6 - Prob. 2CCCh. 27.6 - Prob. 3CCCh. 27 - Describe features of prokaryotes that enable them...Ch. 27 - Mutations are rare and prokaryotes reproduce...Ch. 27 - Describe the range of prokaryotic metabolic...Ch. 27 - How have molecular data informed prokaryotic...Ch. 27 - In what ways are prokaryotes key to the survivaI...Ch. 27 - Prob. 27.6CRCh. 27 - Genetic variation in bacterial populations cannot...Ch. 27 - Photoautotrophs use (A) light as an energy source...Ch. 27 - Which of the following statements is not true? (A)...Ch. 27 - Prob. 4TYUCh. 27 - Prob. 5TYUCh. 27 - Prob. 6TYUCh. 27 - Prob. 7TYUCh. 27 - SCIENTIFIC INQUIRY INTERPRET THE DATA The...Ch. 27 - WRITE ABOUT A THEME: ENERGY In a short essay...Ch. 27 - SYNTHESIZE YOUR KNOWLEDGE Explain how the small...
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- Aside from the bacterial chromosomewhere may DNA be found in a prokaryotic cell? what ways is genetic material transferred from one bacterial cell to another?arrow_forwardUnder the endosymbiotic theory for the origin of the eukaryotic cell. The ancestor of mitochondria and chloroplasts original became part of mutualism with a larger host cell. Which of the following best explains this mutualism? A) The mitochondria and chloroplasts provided specialized reproduction as part of the proto-germ line while the larger host cell provided energy for growth and reproduction. B) The mitochondria and chloroplasts provided gene products that could be used by the larger host cell for improved parasitization. C) The mitochondria and chloroplasts provided molecular energy in return for protection by the larger host cell. D) The mitochondria and chloroplasts produce toxins that the larger cell utilized to defend itself from other cells mutually increasing the defense of all those involved.arrow_forwardThe use of nucleotide sequence data to measure genetic variabilityis complicated by the fact that the genes of many eukaryotesare complex in organization and contain 5' and 3' flankingregions as well as introns. Researchers have compared thenucleotide sequence of two cloned alleles of the y-globin gene from asingle individual and found a variation of 1 percent. Those differencesinclude 13 substitutions of one nucleotide for anotherand three short DNA segments that have been inserted in oneallele or deleted in the other. None of the changes takes placein the gene’s exons (coding regions). Why do you think this isso, and should it change our concept of genetic variation?arrow_forward
- List examples of the efects of chromosomal mutations in humans.arrow_forwardBecause bacteria are able to replicate at high rates, they aresubjected to mistakes or mutations being made in their nucleo-tide sequence. For example, the nucleotide sequence in a genecoding for a protein might be AATTGGCCA but because ofmutation might become GGTTGGCCA. How may such a mu-tation become beneficial to the bacterium? How may it becomeharmful?arrow_forwardIn a species of ciliated protist, a segment of genomic DNA is sometimes deleted. The deletion is a genetically programmed reaction associated with cellular mating. A researcher proposes that the DNA is deleted in atype of recombination called site-specific recombination, with the DNA at either end of the segment joined together and the deleted DNA ending up as a circular DNA reaction product. Suggest how the researcher might use the polymerase chain reaction (PCR) to detect the presence of the circular form of the deleted DNA in an extract of the protistarrow_forward
- When 1 million cells of a culture of haploid yeastcarrying a met− auxotrophic mutation were plated onpetri plates lacking methionine (Met), five coloniesgrew. You would expect cells in which the originalmet− mutation was reversed (by a base change back tothe original sequence) would grow on the media lackingmethionine, but some of these apparent reversions couldbe due to a mutation in a different gene that somehowsuppresses the original met− mutations. How wouldyou be able to determine if the mutations in your fivecolonies were due either to a precise reversion of theoriginal met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?arrow_forwardWhat are the mechanisms by which prokaryotes exchange genetic material via horizontal gene transfer? Describe the three processes.arrow_forwardBacterial endospores form when the environment is no longer conducive to active cell metabolism. The soil bacterium Bacillus subtilis, for example, begins the process of sporulation when one or more nutrients aredepleted. The end product is a small, metabolically dormant structure that can survive almost indefinitely with no detectable metabolism. Spores have mechanisms to prevent accumulation of potentially lethal mutations in their DNA over periods of dormancy that can exceed 1,000 years. B. subtilis spores are much more resistant than are the organism’s growing cells to heat, UV radiation, and oxidizing agents, all of which promote mutations.(a) One factor that prevents potential DNA damage in spores is their greatly decreased water content. How would this affect some types of mutations?(b) Endospores have a category of proteins called small acid-soluble proteins (SASPs) that bind to their DNA, preventing formation of cyclobutane-type dimers. What causes cyclobutane dimers, and why…arrow_forward
- Aside from the bacterial chromosome where may DNA be found in a prokaryotic cell and what ways is genetic material transferred from one bacterial cell to another?arrow_forwardPrice et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport protein (PA) of Bacillusanthracis, the bacterium that causes anthrax in humans. Withinthe 2294-nucleotide gene in 26 strains they identified five pointmutations—two missense and three synonyms—among differentisolates. Necropsy samples from an anthrax outbreak in 1979revealed a novel missense mutation and five unique nucleotidechanges among ten victims. The authors concluded that thesedata indicate little or no horizontal transfer between differentB. anthracis strains. Question: On what basis did the authors conclude that evidence ofhorizontal transfer is absent from their data?arrow_forwardPrice et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport protein (PA) of Bacillusanthracis, the bacterium that causes anthrax in humans. Withinthe 2294-nucleotide gene in 26 strains they identified five pointmutations—two missense and three synonyms—among differentisolates. Necropsy samples from an anthrax outbreak in 1979revealed a novel missense mutation and five unique nucleotidechanges among ten victims. The authors concluded that thesedata indicate little or no horizontal transfer between differentB. anthracis strains. Question: What is meant by ”horizontal transfer”?arrow_forward
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