The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years. Introduction: The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

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Answer 1

Question

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Answer 2

Question

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

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Answer 3

Question

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

Expert Solution & Answer

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Answer 4

Question

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

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Answer 5

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

Answer 6

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Answer 7

Chapter 27, Problem 2AC

Summary Introduction

To determine:

The two species that have been listed as endangered: one that has recovered to the extent that it has been “delisted” and one that has remained on the list for more than 20 years.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act was formed.

Answer 8

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

Answer 9

Summary Introduction

To determine:

The characteristics of two species and their environment have significantly contributed to their differing fates.

Introduction:

The endangered species are those species whose numbers are getting low. To prevent the extinction of those species, the Endangered Species Act is formed. The species which are at a risk of extinction are a salamander, blue whale, and snow leopard. The species which are delisted from the list of endangered species are Caribbean monk seal and Canary rockfish.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 27.1 - Prob. 1CSC Ch. 27.1 - Prob. 1CYL Ch. 27.1 - Prob. 1HYEW Ch. 27.1 - The Return of the Elephant Seals Female elephant...Ch. 27.1 - Prob. 2CYL Ch. 27.1 - Prob. 3CYL Ch. 27.2 - Prob. 1CSC Ch. 27.2 - Prob. 1CYL Ch. 27.2 - Prob. 1TC Ch. 27.2 - Prob. 2CYL

Solution Summary: The author explains that the Endangered Species Act protects endangered species from being extinct.

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