Chapter 27, Problem 27.62AP

Interpretation Introduction

(a)

Interpretation:

The explanation for iodoacetic acid reaction with side chain thiol group of a cysteine residue is to be stated. The reason as to why this reaction is necessary before sequencing is to be stated.

Concept introduction:

The reagent DTT is known as dithiothreitol and is a common redox reagent. It is also known as Cleland’s reagent. It is relatively not a stable compound due to air oxidation. It is used as a deprotecting group and to break the disulfide bond in protein.

Interpretation Introduction

(b)

Interpretation:

The product obtained on oxidation of disulfide bond with ${\text{H}}_{\text{2}}{\text{O}}_{\text{2}}$ that accomplishes the same objective is to be stated.

Concept introduction:

The compound hydrogen peroxide represented by ${\text{H}}_{\text{2}}{\text{O}}_{\text{2}}$ is more viscous than water. It is used as an oxidizer, antiseptic, bleaching agent, and to break the disulfide bond in protein. It is an unstable compound and it decomposes in the presence of light.