Laboratory Experiments in Microbiology (11th Edition)
Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 27, Problem 1Q

Write the chemical reaction catalyzed by nitrate reductase.

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Summary Introduction

To write:

The chemical reaction catalyzed by nitrate reductase

Introduction:

Catalysis is the process that raises the rate of a chemical reaction by adding an element known as a catalyst. Catalyst is not utilized in the reaction. The catalyst usually forms a temporary intermediate product.

Chemical reactions occur rapidly in the presence (or existence) of a catalyst because the catalyst gives an alternative reaction pathway with lower activation energy than the non-catalyzed mechanism.

In the presence (or existence) of a catalyst, less free energy is needed to reach the transition state, but the total free energy to the products from the reactants does not change. As compared to the non-catalyzed reaction, catalyzed reactions have lower activation energy.

Explanation of Solution

Nitrate reductase is molybdenoenzymes. It is responsible for catalyzing the nitrate assimilation (rate-limiting step). It is not only accountable for catalyzing, but also increases the production of amino acids in tea leaves.

Nitrogen assimilation forms organic nitrogen compounds (like amino acids) from inorganic nitrogen compounds existing in the atmosphere. The first step of this process is the decrease of nitrate to nitrite in the cytosol. It is catalyzed by nitrate reductase.

  NO3+NAD(P)H+H++2eNO2+NAD(P)++H2O

Conclusion

The chemical reaction catalyzed by nitrate reductase is nitrogen assimilation.

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