Chapter 26.SE, Problem 46AP

Interpretation Introduction

Interpretation:

The amide bonds that are cleaved by trypsin and by chymotrypsin in the polypeptide, Phe-Leu-Met-Lys-Tyr-Asp-Gly-Arg-Val-Ile-Pro-Tyr, are to be given.

Concept introduction:

Partial hydrolysis of a peptide can be carried out using enzymes which are specific in their action. The enzyme trypsin catalyses the hydrolysis of peptides only at the carboxyl side of the basic amino acids arginine ald lysine. The enzyme chymotrypsin cleaves only at the carboxyl side of the aryl substituted amino acids phenylalanine, tyrosin and tryptophan.

To give:

The amide bonds that are cleaved by trypsin and by chymotrypsin in the polypeptide, Phe-Leu-Met-Lys-Tyr-Asp-Gly-Arg-Val-Ile-Pro-Tyr.