The negative absorbance of all the peaks that obtained from ion-exchange chromatography in the study of decomposition of dithionite should be explained. Ion-Exchange Chromatography: Ion-Exchange Chromatography is separation technique, which is work in the principle of exchanging of ions based on attraction to the ion exchanger. It contains two phases, one is stationary phase and another one is mobile phase. In generally resins are act as a stationary phase, the positively charged ion exchangers attract solute anions and negatively charged ion exchangers are attract solute cations. The higher polar eluent is passed through a column the exchangers are releases the solute and they will come out from the column. In this process, the stationary phase (ion exchangers) is exchange the solute ions into eluent ions therefore it is called as Ion-exchange chromatography. Indirect detection: In the separation ions in ion-exchange chromatography, the separated anion or cation are detected by addition of substance which is have a steady background signal with the background electrolyte, the signal of the additional substance are remove from the chromatogram to give a signal of sample. In this method the sample is detected by negative signals of additional substance so this is called as an indirect detection.

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Chapter 26, Problem 26.16P

Interpretation Introduction

Interpretation:

The negative absorbance of all the peaks that obtained from ion-exchange chromatography in the study of decomposition of dithionite should be explained.

Ion-Exchange Chromatography:

Ion-Exchange Chromatography is separation technique, which is work in the principle of exchanging of ions based on attraction to the ion exchanger.

It contains two phases, one is stationary phase and another one is mobile phase.

In generally resins are act as a stationary phase, the positively charged ion exchangers attract solute anions and negatively charged ion exchangers are attract solute cations.

The higher polar eluent is passed through a column the exchangers are releases the solute and they will come out from the column.

In this process, the stationary phase (ion exchangers) is exchange the solute ions into eluent ions therefore it is called as Ion-exchange chromatography.

Indirect detection:

In the separation ions in ion-exchange chromatography, the separated anion or cation are detected by addition of substance which is have a steady background signal with the background electrolyte, the signal of the additional substance are remove from the chromatogram to give a signal of sample.

In this method the sample is detected by negative signals of additional substance so this is called as an indirect detection.

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