Chapter 25.11, Problem 14P

Interpretation Introduction

Interpretation:

The labeled carbon atoms of cholesterol when acetate enriched with ${}^{\text{14}}\text{C}$ in its methyl group $\left({}^{\text{14}}{\text{CH}}_{\text{3}}\text{COOH}\right)$ is used as the carbon source are to be identified.

Concept introduction:

The isotopically labeled carbons is incorporated in various compounds by their biological enrichment with ${}^{\text{14}}\text{C}$ isotope in the methyl group which are labeled at carbons $2,4,6,8$ and so on, also at the carbon atoms of the branched methyl groups in the structure.