ANATOMY+PHYSIOLOGY >LOOSE<
8th Edition
ISBN: 9781308329826
Author: SALADIN
Publisher: MCG/CREATE
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Chapter 25.1, Problem 4BYGO
Summary Introduction
Introduction:
The dietary macromolecules that are broken down into monomers by a series of hydrolysis reactions is a chemical digestion i.e., polysaccharides into monosaccharides, proteins are broken down into amino acids, fats into fatty acid and monoglycerides, and
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 25 Solutions
ANATOMY+PHYSIOLOGY >LOOSE<
Ch. 25.1 - Prob. 1BYGOCh. 25.1 - Prob. 2BYGOCh. 25.1 - Prob. 3BYGOCh. 25.1 - Prob. 4BYGOCh. 25.1 - Prob. 1AYLOCh. 25.1 - The difference between the digestive tract and the...Ch. 25.1 - Prob. 3AYLOCh. 25.1 - Prob. 4AYLOCh. 25.1 - Prob. 5AYLOCh. 25.1 - Prob. 6AYLO
Ch. 25.2 - Prob. 5BYGOCh. 25.2 - Prob. 6BYGOCh. 25.2 - Prob. 7BYGOCh. 25.2 - Prob. 8BYGOCh. 25.2 - Prob. 9BYGOCh. 25.2 - Seven functions of the oral cavityCh. 25.2 - Prob. 2AYLOCh. 25.2 - Prob. 3AYLOCh. 25.2 - Prob. 4AYLOCh. 25.2 - Anatomy of the hard and soft palates; the two...Ch. 25.2 - Prob. 6AYLOCh. 25.2 - Six functions of saliva; its composition and pH;...Ch. 25.2 - Prob. 8AYLOCh. 25.2 - Prob. 9AYLOCh. 25.2 - The physiology of swallowing; the swallowing...Ch. 25.3 - Prob. 10BYGOCh. 25.3 - Prob. 11BYGOCh. 25.3 - Prob. 12BYGOCh. 25.3 - Prob. 13BYGOCh. 25.3 - Anatomy and functions of the stomach; features...Ch. 25.3 - Prob. 2AYLOCh. 25.3 - Prob. 3AYLOCh. 25.3 - Prob. 4AYLOCh. 25.3 - The cells that secrete hydrochloric acid, how they...Ch. 25.3 - Prob. 6AYLOCh. 25.3 - Prob. 7AYLOCh. 25.3 - Prob. 8AYLOCh. 25.3 - Prob. 9AYLOCh. 25.3 - Prob. 10AYLOCh. 25.3 - Prob. 11AYLOCh. 25.3 - The degree of digestion that occurs in the...Ch. 25.3 - Prob. 13AYLOCh. 25.3 - Prob. 14AYLOCh. 25.4 - Prob. 14BYGOCh. 25.4 - Prob. 15BYGOCh. 25.4 - Prob. 16BYGOCh. 25.4 - Prob. 17BYGOCh. 25.4 - Prob. 1AYLOCh. 25.4 - Prob. 2AYLOCh. 25.4 - Prob. 3AYLOCh. 25.4 - Prob. 4AYLOCh. 25.4 - Prob. 5AYLOCh. 25.4 - Prob. 6AYLOCh. 25.4 - Prob. 7AYLOCh. 25.4 - Composition and digestive functions of pancreatic...Ch. 25.4 - Prob. 9AYLOCh. 25.5 - Prob. 18BYGOCh. 25.5 - Prob. 19BYGOCh. 25.5 - Distinguish between segmentation and the migrating...Ch. 25.5 - Structures that mark the beginning and end of the...Ch. 25.5 - Prob. 2AYLOCh. 25.5 - Prob. 3AYLOCh. 25.5 - Prob. 4AYLOCh. 25.5 - Prob. 5AYLOCh. 25.5 - Prob. 6AYLOCh. 25.6 - What three classes of nutrients are most abundant?...Ch. 25.6 - Prob. 22BYGOCh. 25.6 - Prob. 23BYGOCh. 25.6 - Prob. 24BYGOCh. 25.6 - Prob. 25BYGOCh. 25.6 - Prob. 1AYLOCh. 25.6 - Prob. 2AYLOCh. 25.6 - Prob. 3AYLOCh. 25.6 - Prob. 4AYLOCh. 25.6 - Prob. 5AYLOCh. 25.6 - Prob. 6AYLOCh. 25.6 - Prob. 7AYLOCh. 25.6 - Differences between emulsification droplets,...Ch. 25.6 - Prob. 9AYLOCh. 25.6 - Prob. 10AYLOCh. 25.6 - Prob. 11AYLOCh. 25.7 - Prob. 26BYGOCh. 25.7 - Prob. 27BYGOCh. 25.7 - Prob. 28BYGOCh. 25.7 - Prob. 1AYLOCh. 25.7 - Prob. 2AYLOCh. 25.7 - Prob. 3AYLOCh. 25.7 - Mechanisms of the intrinsic and parasympathetic...Ch. 25 - Prob. 1TYRCh. 25 - Prob. 2TYRCh. 25 - Prob. 3TYRCh. 25 - Prob. 4TYRCh. 25 - Prob. 5TYRCh. 25 - All of the following contribute to the absorptive...Ch. 25 - Which of the following is a periodontal tissue? a....Ch. 25 - Prob. 8TYRCh. 25 - Prob. 9TYRCh. 25 - Prob. 10TYRCh. 25 - Cusps are a feature of the ______ surfaces of the...Ch. 25 - Prob. 12TYRCh. 25 - Prob. 13TYRCh. 25 - Prob. 14TYRCh. 25 - Nervous stimulation of gastrointestinal activity...Ch. 25 - Prob. 16TYRCh. 25 - Prob. 17TYRCh. 25 - Prob. 18TYRCh. 25 - Prob. 19TYRCh. 25 - Prob. 20TYRCh. 25 - Prob. 1BYMVCh. 25 - Prob. 2BYMVCh. 25 - -elleCh. 25 - Prob. 4BYMVCh. 25 - Prob. 5BYMVCh. 25 - Prob. 6BYMVCh. 25 - Prob. 7BYMVCh. 25 - porto-Ch. 25 - Prob. 9BYMVCh. 25 - Prob. 10BYMVCh. 25 - Prob. 1WWTSCh. 25 - Prob. 2WWTSCh. 25 - Prob. 3WWTSCh. 25 - Prob. 4WWTSCh. 25 - Lipids enter the circulation when the intestinal...Ch. 25 - Prob. 6WWTSCh. 25 - Prob. 7WWTSCh. 25 - Prob. 8WWTSCh. 25 - Prob. 9WWTSCh. 25 - Prob. 10WWTSCh. 25 - Prob. 1TYCCh. 25 - Prob. 2TYCCh. 25 - What do carboxypeptidase and aminopeptidase have...Ch. 25 - What do micelles and chylomicrons have in common?...Ch. 25 - Explain why most dietary lipids must be absorbed...
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