ANATOMY+PHYSIOLOGY >LOOSE<
8th Edition
ISBN: 9781308329826
Author: SALADIN
Publisher: MCG/CREATE
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Chapter 25.6, Problem 24BYGO
Summary Introduction
Introduction:
Lipids are hydrophobic in nature, so digestion and absorption of lipid is a more complicated process when compared to protein and carbohydrate digestion. Lingual lipase in the mouth is secreted by intrinsic salivary glands that digest small quantity of fat; this enzyme is more active in the stomach because of acidic pH. Emulsification droplets, micelles, and chylomicron are involved in lipid digestion.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 25 Solutions
ANATOMY+PHYSIOLOGY >LOOSE<
Ch. 25.1 - Prob. 1BYGOCh. 25.1 - Prob. 2BYGOCh. 25.1 - Prob. 3BYGOCh. 25.1 - Prob. 4BYGOCh. 25.1 - Prob. 1AYLOCh. 25.1 - The difference between the digestive tract and the...Ch. 25.1 - Prob. 3AYLOCh. 25.1 - Prob. 4AYLOCh. 25.1 - Prob. 5AYLOCh. 25.1 - Prob. 6AYLO
Ch. 25.2 - Prob. 5BYGOCh. 25.2 - Prob. 6BYGOCh. 25.2 - Prob. 7BYGOCh. 25.2 - Prob. 8BYGOCh. 25.2 - Prob. 9BYGOCh. 25.2 - Seven functions of the oral cavityCh. 25.2 - Prob. 2AYLOCh. 25.2 - Prob. 3AYLOCh. 25.2 - Prob. 4AYLOCh. 25.2 - Anatomy of the hard and soft palates; the two...Ch. 25.2 - Prob. 6AYLOCh. 25.2 - Six functions of saliva; its composition and pH;...Ch. 25.2 - Prob. 8AYLOCh. 25.2 - Prob. 9AYLOCh. 25.2 - The physiology of swallowing; the swallowing...Ch. 25.3 - Prob. 10BYGOCh. 25.3 - Prob. 11BYGOCh. 25.3 - Prob. 12BYGOCh. 25.3 - Prob. 13BYGOCh. 25.3 - Anatomy and functions of the stomach; features...Ch. 25.3 - Prob. 2AYLOCh. 25.3 - Prob. 3AYLOCh. 25.3 - Prob. 4AYLOCh. 25.3 - The cells that secrete hydrochloric acid, how they...Ch. 25.3 - Prob. 6AYLOCh. 25.3 - Prob. 7AYLOCh. 25.3 - Prob. 8AYLOCh. 25.3 - Prob. 9AYLOCh. 25.3 - Prob. 10AYLOCh. 25.3 - Prob. 11AYLOCh. 25.3 - The degree of digestion that occurs in the...Ch. 25.3 - Prob. 13AYLOCh. 25.3 - Prob. 14AYLOCh. 25.4 - Prob. 14BYGOCh. 25.4 - Prob. 15BYGOCh. 25.4 - Prob. 16BYGOCh. 25.4 - Prob. 17BYGOCh. 25.4 - Prob. 1AYLOCh. 25.4 - Prob. 2AYLOCh. 25.4 - Prob. 3AYLOCh. 25.4 - Prob. 4AYLOCh. 25.4 - Prob. 5AYLOCh. 25.4 - Prob. 6AYLOCh. 25.4 - Prob. 7AYLOCh. 25.4 - Composition and digestive functions of pancreatic...Ch. 25.4 - Prob. 9AYLOCh. 25.5 - Prob. 18BYGOCh. 25.5 - Prob. 19BYGOCh. 25.5 - Distinguish between segmentation and the migrating...Ch. 25.5 - Structures that mark the beginning and end of the...Ch. 25.5 - Prob. 2AYLOCh. 25.5 - Prob. 3AYLOCh. 25.5 - Prob. 4AYLOCh. 25.5 - Prob. 5AYLOCh. 25.5 - Prob. 6AYLOCh. 25.6 - What three classes of nutrients are most abundant?...Ch. 25.6 - Prob. 22BYGOCh. 25.6 - Prob. 23BYGOCh. 25.6 - Prob. 24BYGOCh. 25.6 - Prob. 25BYGOCh. 25.6 - Prob. 1AYLOCh. 25.6 - Prob. 2AYLOCh. 25.6 - Prob. 3AYLOCh. 25.6 - Prob. 4AYLOCh. 25.6 - Prob. 5AYLOCh. 25.6 - Prob. 6AYLOCh. 25.6 - Prob. 7AYLOCh. 25.6 - Differences between emulsification droplets,...Ch. 25.6 - Prob. 9AYLOCh. 25.6 - Prob. 10AYLOCh. 25.6 - Prob. 11AYLOCh. 25.7 - Prob. 26BYGOCh. 25.7 - Prob. 27BYGOCh. 25.7 - Prob. 28BYGOCh. 25.7 - Prob. 1AYLOCh. 25.7 - Prob. 2AYLOCh. 25.7 - Prob. 3AYLOCh. 25.7 - Mechanisms of the intrinsic and parasympathetic...Ch. 25 - Prob. 1TYRCh. 25 - Prob. 2TYRCh. 25 - Prob. 3TYRCh. 25 - Prob. 4TYRCh. 25 - Prob. 5TYRCh. 25 - All of the following contribute to the absorptive...Ch. 25 - Which of the following is a periodontal tissue? a....Ch. 25 - Prob. 8TYRCh. 25 - Prob. 9TYRCh. 25 - Prob. 10TYRCh. 25 - Cusps are a feature of the ______ surfaces of the...Ch. 25 - Prob. 12TYRCh. 25 - Prob. 13TYRCh. 25 - Prob. 14TYRCh. 25 - Nervous stimulation of gastrointestinal activity...Ch. 25 - Prob. 16TYRCh. 25 - Prob. 17TYRCh. 25 - Prob. 18TYRCh. 25 - Prob. 19TYRCh. 25 - Prob. 20TYRCh. 25 - Prob. 1BYMVCh. 25 - Prob. 2BYMVCh. 25 - -elleCh. 25 - Prob. 4BYMVCh. 25 - Prob. 5BYMVCh. 25 - Prob. 6BYMVCh. 25 - Prob. 7BYMVCh. 25 - porto-Ch. 25 - Prob. 9BYMVCh. 25 - Prob. 10BYMVCh. 25 - Prob. 1WWTSCh. 25 - Prob. 2WWTSCh. 25 - Prob. 3WWTSCh. 25 - Prob. 4WWTSCh. 25 - Lipids enter the circulation when the intestinal...Ch. 25 - Prob. 6WWTSCh. 25 - Prob. 7WWTSCh. 25 - Prob. 8WWTSCh. 25 - Prob. 9WWTSCh. 25 - Prob. 10WWTSCh. 25 - Prob. 1TYCCh. 25 - Prob. 2TYCCh. 25 - What do carboxypeptidase and aminopeptidase have...Ch. 25 - What do micelles and chylomicrons have in common?...Ch. 25 - Explain why most dietary lipids must be absorbed...
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