The reactions underlying the observations that when 32 P-labeled DNA and unlabeled dNTPs are added, 32 PdNMPs are not released. Introduction : DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

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Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…

Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following observations and experiments. (a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and 53.29% O by weight. (b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u). (c) Infrared spectroscopy showed that X contained one double bond. (d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter. (e) The aqueous solution of X is acidic. What is the empirical formula of X?

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Answer 2

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Answer 3

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Expert Solution & Answer

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Answer 4

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Expert Solution & Answer

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Answer 5

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 6

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Answer 7

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Answer 8

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 9

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 25 - Prob. 1P Ch. 25 - Prob. 2P Ch. 25 - Prob. 3P Ch. 25 - Prob. 4P Ch. 25 - Prob. 5P Ch. 25 - Prob. 6P Ch. 25 - Prob. 7P Ch. 25 - Prob. 8P Ch. 25 - Prob. 9P Ch. 25 - Prob. 10P

Concept explainers

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Chapter 25 Solutions

Concept explainers

To determine: What is expected if pyrophosphate is added instead of dNTPs . Introduction: dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Chapter 25 Solutions

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.