Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp. Introduction: Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

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Answer 1

Question

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

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You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

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Answer 2

Question

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Expert Solution & Answer

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See solution

Answer 4

Question

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Expert Solution & Answer

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See solution

Answer 5

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Answer 6

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

Answer 7

Chapter 25, Problem 3P

(a)

Summary Introduction

To determine: Turns of the double helix that must be unwound during replication of the E.coli chromosome if total base pairs in E.coli are 4,641,652 bp.

Introduction:

Replication model of DNA is semi-conservative where one strand in the next generation has a light band labeling and one strand has a dark band labeling of N-14 and N-15 atoms respectively.

Answer 8

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

Answer 9

(b)

Summary Introduction

To determine: Time taken to replicate the E.coli chromosome at 37°C, if two replication forks proceeded from origin. It is assumed that replication occurs at a rate of 1000 bp/sec with assumption that E.coli cells divide every 20 min.

Introduction:

Replication forks are formed during the stage of synthesis of DNA. It involves the bi-directional method of replication of DNA with formation of a leading and a lagging strand of DNA.

Answer 10

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

Answer 11

(c1)

Summary Introduction

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

Answer 12

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Answer 13

(c2)

Summary Introduction

To determine: The factors that determine the numerous Okazaki fragments are assembled in the correct order in the new DNA.

Introduction:

The process of DNA replication synthesis involves the formation of leading and lagging strands. Lagging strand with the addition of the Okazaki fragments synthesizes the new template with the help of enzyme DNA polymerase.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 25 - Prob. 1P Ch. 25 - Prob. 2P Ch. 25 - Prob. 3P Ch. 25 - Prob. 4P Ch. 25 - Prob. 5P Ch. 25 - Prob. 6P Ch. 25 - Prob. 7P Ch. 25 - Prob. 8P Ch. 25 - Prob. 9P Ch. 25 - Prob. 10P

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To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

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Chapter 25 Solutions

Concept explainers

Videos

To determine: The number of Okazaki fragments formed after replication of E.coli. Introduction: Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.

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Chapter 25 Solutions

To determine: The number of Okazaki fragments formed after replication of E.coli.

Introduction:

Okazaki fragments are small nucleotides segments of DNA which bind to the complementary segments on single stranded DNA lagging strand during the process of DNA synthesis.