Chapter 25, Problem 9P

Summary Introduction

To explain: The formation of two radioactive bands one with a high molecular weight and other with a low molecular weight when the E. coli mutants exposed to $\text{H}$-labeled thymine are sedimented on an alkaline sucrose density gradient..

Introduction:

$\text{DNA}$ ligase enzyme forms phosphodiester bonds and seals the bonds between various nucleotides leading to the formation of double strand. It requires the energy derived from nucleotide $\text{ATP}$ inside the cell.