The initial PTH derivative formed when Ala-Phe-Val undergoes Edman degradation need to be drawn. Concept introduction: Edman degradation is a chemical reaction where the N-terminus amino acid is removed when the peptide is treated with phenyl isothiocyanate followed by treatment with trifluoroacetic acid to form phenylthiohydantoin(PTH) derivative and peptide with one amino acid residue less than the starting peptide. This method can be used to identify the sequence of the amino acid in peptide as only one amino acid is removed each time forming the PTH derivative. The reaction takes place because the amino group is sufficiently nucleophilic to attack phenyl isothiocyanate. The general scheme for Edman degradation can be shown as, Please remember for Edman degradation reaction to take place the N-terminal of the peptides under consideration should be free .

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Chapter 25, Problem 75PP

Interpretation Introduction

Interpretation:

The initial PTH derivative formed when Ala-Phe-Val undergoes Edman degradation need to be drawn.

Concept introduction:

Edman degradation is a chemical reaction where the N-terminus amino acid is removed when the peptide is treated with phenyl isothiocyanate followed by treatment with trifluoroacetic acid to form phenylthiohydantoin(PTH) derivative and peptide with one amino acid residue less than the starting peptide. This method can be used to identify the sequence of the amino acid in peptide as only one amino acid is removed each time forming the PTH derivative. The reaction takes place because the amino group is sufficiently nucleophilic to attack phenyl isothiocyanate. The general scheme for Edman degradation can be shown as,

Please remember for Edman degradation reaction to take place the N-terminal of the peptides under consideration should be free.

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I have a question about this problem involving mechanisms and drawing curved arrows for acids and bases. I know we need to identify the nucleophile and electrophile, but are there different types of reactions? For instance, what about Grignard reagents and other types that I might not be familiar with? Can you help me with this? I want to identify the names of the mechanisms for problems 1-14, such as Gilman reagents and others. Are they all the same? Also, could you rewrite it so I can better understand? The handwriting is pretty cluttered. Additionally, I need to label the nucleophile and electrophile, but my main concern is whether those reactions differ, like the "Brønsted-Lowry acid-base mechanism, Lewis acid-base mechanism, acid-catalyzed mechanisms, acid-catalyzed reactions, base-catalyzed reactions, nucleophilic substitution mechanisms (SN1 and SN2), elimination reactions (E1 and E2), organometallic mechanisms, and so forth."