EP HUMAN ANATOMY+PHYSIOLOGY-MOD.MASTER.
2nd Edition
ISBN: 9780134788067
Author: AMERMAN
Publisher: PEARSON CO
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Chapter 25, Problem 3AYKA
Summary Introduction
Case summary:
Ms. Johanssen, a patient in the hospital, developed hyperkalemia and acidosis. On examining her medical chart, it is noticed that she has been prescribed a double dose of spironolactone.
Characters in the case:
Ms. Johanssen, having hyperkalemia and acidosis.
Adequate information:
Ms. Johanssen has developed hyperkalemia and acidosis, and her spironolactone dose has been doubled.
To determine:
The correlation between the laboratory findings and the doubled dose of spironolactone.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 25 Solutions
EP HUMAN ANATOMY+PHYSIOLOGY-MOD.MASTER.
Ch. 25.1 - 1. What is a body fluid?
Ch. 25.1 - 2. What is balance with respect to body fluids?
Ch. 25.1 - How does an electrolyte differ from a...Ch. 25.1 - What is electrolyte balance?Ch. 25.1 - 5. How do acids and bases differ?
Ch. 25.1 - 6. Which pH values are acidic, basic, and...Ch. 25.2 - Prob. 1QCCh. 25.2 - What factors affect total body water?Ch. 25.2 - 3. Where are the intracellular and extracellular...Ch. 25.2 - Prob. 4QC
Ch. 25.2 - Prob. 5QCCh. 25.2 - Prob. 6QCCh. 25.2 - Prob. 7QCCh. 25.2 - How is thirst stimulated?Ch. 25.2 - How are fluids lost from the body?Ch. 25.2 - 10. What are the water requirements for an...Ch. 25.2 - 11. What is the role of ADH in fluid balance?
Ch. 25.2 - How is ADH secretion stimulated?Ch. 25.2 - How does dehydration affect the volume of the...Ch. 25.2 - Prob. 14QCCh. 25.2 - 15. How do dehydration and overhydration differ...Ch. 25.3 - What are the main roles of sodium ions in the...Ch. 25.3 - How is sodium ion concentration regulated?Ch. 25.3 - Prob. 3QCCh. 25.3 - Prob. 4QCCh. 25.3 - 5. How is the concentration of potassium ions in...Ch. 25.3 - 6. What happens to the resting membrane potential...Ch. 25.3 - Prob. 7QCCh. 25.3 - Prob. 8QCCh. 25.3 - Prob. 9QCCh. 25.3 - Prob. 10QCCh. 25.3 - 11. How is chloride ion reabsorption in the...Ch. 25.3 - 12. How is the concentration of magnesium ions in...Ch. 25.4 - What are the major sources of acids for the body?Ch. 25.4 - Prob. 2QCCh. 25.4 - Prob. 3QCCh. 25.4 - Prob. 4QCCh. 25.4 - Prob. 5QCCh. 25.4 - Prob. 6QCCh. 25.4 - Prob. 7QCCh. 25.4 - Prob. 8QCCh. 25.4 - How do metabolic acidosis and respiratory acidosis...Ch. 25.4 - Prob. 10QCCh. 25.4 - Prob. 11QCCh. 25.5 - Prob. 1QCCh. 25.5 - Prob. 2QCCh. 25 - Prob. 1CYRCh. 25 - 2. How does an electrolyte differ from a...Ch. 25 - Prob. 3CYRCh. 25 - Prob. 4CYRCh. 25 - Prob. 5CYRCh. 25 - Prob. 6CYRCh. 25 - Which of the following is false with respect to...Ch. 25 - Prob. 8CYRCh. 25 - Prob. 9CYRCh. 25 - Prob. 10CYRCh. 25 - Prob. 11CYRCh. 25 - Prob. 12CYRCh. 25 - Prob. 13CYRCh. 25 - Prob. 14CYRCh. 25 - 15. Which of the following mechanisms is/are used...Ch. 25 - Mark the following statements as true or false. If...Ch. 25 - Prob. 17CYRCh. 25 - 18. How does angiotensin-II help to restore fluid...Ch. 25 - Prob. 1CYUCh. 25 - Prob. 2CYUCh. 25 - Prob. 3CYUCh. 25 - Prob. 4CYUCh. 25 - Prob. 1AYKACh. 25 - Prob. 2AYKACh. 25 - Prob. 3AYKACh. 25 - Prob. 4AYKACh. 25 - Prob. 5AYKB
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