Chapter 24.5, Problem 2.3ACP

(a)

Interpretation Introduction

Interpretation:

The primers frequently chosen that have high number of cytosines and guanines in their sequences; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand($\text{18-20}$ base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

(b)

Interpretation Introduction

Interpretation:

The primer sequence of CCCCCCCCCCGGGGGGGGGG not is a good choice; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand($\text{18-20}$ base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

(c)

Interpretation Introduction

Interpretation:

Vast excess of primer used for the amount of DNA has to be amplified.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand($\text{18-20}$ base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.