The trial in which the most control crabs and experimental bled crabs died. Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Question

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Answer 1

Question

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

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Answer 3

Question

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

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Answer 4

Question

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

Answer 6

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.

Conclusion

Conclusion: Trail group 5 marked the highest number of control crabs and bled crabs that died compared to the other trial groups.

Answer 7

Chapter 24, Problem 1DAA

Summary Introduction

To explain: The trial in which the most control crabs and experimental bled crabs died.

Concept introduction: Horseshoe crab belongs to Phylum: Arthropod. They are normally found around shallow ocean waters. Horseshoe crabs are living fossils. Though the horseshoe crabs look like crustaceans, they are marine arachnids.

Answer 8

Expert Solution & Answer

Explanation of Solution

The uniqueness of horseshoe crab is that they clot upon encountering bacteria. Therefore, they are used in experiments where highly virulent bacteria are involved. The blood of horseshoe crabs is taken and then, they are let out in their habitat in order to conserve their population, but this might also lead to their death. Therefore, experiments were conducted to check their survival after taking their blood and the results were obtained as seen in Fig.24.40 in the question.

In trial 5, the number of control crabs is 30; out of which, 1 died. In trial 5, the number of bled animals is 30; out of which, 6 died.