The reason why σ = 0 lane in gel A has multiple bands. Introduction: Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Question

Want to see the full answer?

Answer 1

Question

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

(f) SO: Best Lewis Structure 3 e group geometry:_ shape/molecular geometry:, (g) CF2CF2 Best Lewis Structure polarity: e group arrangement:_ shape/molecular geometry: (h) (NH4)2SO4 Best Lewis Structure polarity: e group arrangement: shape/molecular geometry: polarity: Sketch (with angles): Sketch (with angles): Sketch (with angles):

1. Problem Set 3b Chem 141 For each of the following compounds draw the BEST Lewis Structure then sketch the molecule (showing bond angles). Identify (i) electron group geometry (ii) shape around EACH central atom (iii) whether the molecule is polar or non-polar (iv) (a) SeF4 Best Lewis Structure e group arrangement:_ shape/molecular geometry: polarity: (b) AsOBr3 Best Lewis Structure e group arrangement:_ shape/molecular geometry: polarity: Sketch (with angles): Sketch (with angles):

(c) SOCI Best Lewis Structure 2 e group arrangement: shape/molecular geometry:_ (d) PCls Best Lewis Structure polarity: e group geometry:_ shape/molecular geometry:_ (e) Ba(BrO2): Best Lewis Structure polarity: e group arrangement: shape/molecular geometry: polarity: Sketch (with angles): Sketch (with angles): Sketch (with angles):

Answer 2

Question

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 6

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 7

Chapter 24, Problem 15P

(a)

Summary Introduction

To describe: The reason why σ = 0 lane in gel A has multiple bands.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 8

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 9

(b)

Summary Introduction

To determine: In gel B, whether the DNA from the σ = 0 preparation positively or negatively supercoiled in the presence of intercalating dye.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 10

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 11

(c)

Summary Introduction

To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the σ = −0.115 lane has two bands, one highly supercoiled DNA and one relaxed.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 12

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 13

(d)

Summary Introduction

To determine: The approximate superhelical density of the native DNA.

Introduction:

Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.

Answer 14

Expert Solution & Answer

Answer 15

Expert Solution & Answer

Answer 16

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 17

Ch. 24 - Prob. 1P Ch. 24 - Prob. 2P Ch. 24 - Prob. 3P Ch. 24 - Prob. 4P Ch. 24 - Prob. 5P Ch. 24 - Prob. 6P Ch. 24 - Prob. 7P Ch. 24 - Prob. 8P Ch. 24 - Prob. 9P Ch. 24 - Prob. 10P

Concept explainers

Videos

Want to see the full answer?

Chapter 24 Solutions