EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 24, Problem 7P
Summary Introduction
To determine: The superhelical density (σ) for the plasmid DNA isolated from E. coli and the possibilities that bacteriophages λ will be able to infect the E. coli culture.
Introduction:
In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. A quantity which is independent of the length of DNA molecule is termed as “superhelical density or specific linking number” (σ). It can define the change in linking number. It is a measurement of the numbers of turns, eliminated than the number of turns present in relaxed DNA.
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9. Which one of the compounds below is the major organic product obtained from
the following reaction sequence, starting with ethyl acetoacetate?
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1. NaOCH2CH3
CH3CH2OH
1. NaOH, H₂O
2. H3O+
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OCH2CH3
2.
ethyl acetoacetate
ii
A
3. H3O+
OH
B
C
D
E
7. Only one of the following ketones cannot be made via an acetoacetic ester
synthesis. Which one is it?
Ph
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A
B
C
D
E
Chapter 24 Solutions
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