The functions of the blood. Introduction: Blood refers to the fluid, which travels via the circulatory system’s vessels. In humans, it comprises of the liquid portion, known as plasma, the cells of the blood that comes both in the form of red and white blood cells and the fragments of the cells known as platelets. Each of the constituents of the blood plays an essential and vital role; the deficiency of any one of these can cause havoc in the proper functioning of the human body.

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Chapter 23.3, Problem 1LO

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The functions of the blood.

Introduction:

Blood refers to the fluid, which travels via the circulatory system’s vessels. In humans, it comprises of the liquid portion, known as plasma, the cells of the blood that comes both in the form of red and white blood cells and the fragments of the cells known as platelets. Each of the constituents of the blood plays an essential and vital role; the deficiency of any one of these can cause havoc in the proper functioning of the human body.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…