BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 23.2, Problem 9A
Summary Introduction
Introduction:
Blood pressure is caused by the force of flow of blood in the walls of aorta. The blood pressure is maximum in the capillaries of arteries, while it is lesser in the capillary end of veins.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 23 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 23.1 - Prob. 1LOCh. 23.1 - Prob. 2LOCh. 23.1 - Prob. 3LOCh. 23.1 - Summarize the differences between the pulmonary...Ch. 23.1 - Prob. 1CYPCh. 23.1 - Prob. 2CYPCh. 23.1 - Prob. 3CYPCh. 23.1 - Summarize the difference between the circulatory...Ch. 23.1 - Prob. 1ACh. 23.1 - Prob. 2A
Ch. 23.1 - Prob. 3ACh. 23.2 - Describe the anatomy of the human heart.Ch. 23.2 - Prob. 2LOCh. 23.2 - Prob. 3LOCh. 23.2 - Prob. 4LOCh. 23.2 - Prob. 5LOCh. 23.2 - Prob. 6LOCh. 23.2 - Prob. 1CYPCh. 23.2 - Prob. 2CYPCh. 23.2 - Prob. 3CYPCh. 23.2 - Prob. 4CYPCh. 23.2 - Prob. 5CYPCh. 23.2 - Prob. 4ACh. 23.2 - Prob. 5ACh. 23.2 - Prob. 6ACh. 23.2 - Prob. 7ACh. 23.2 - Prob. 8ACh. 23.2 - Prob. 9ACh. 23.2 - Prob. 10ACh. 23.3 - Prob. 1LOCh. 23.3 - Prob. 2LOCh. 23.3 - Prob. 3LOCh. 23.3 - Prob. 4LOCh. 23.3 - Prob. 5LOCh. 23.3 - Prob. 1CYPCh. 23.3 - Prob. 2CYPCh. 23.3 - Describe the process involved in forming a blood...Ch. 23.3 - Prob. 4CYPCh. 23.3 - Prob. 11ACh. 23.3 - Prob. 12ACh. 23.3 - Prob. 13ACh. 23.3 - Prob. 14ACh. 23.3 - Which of the following are not involved in blood...Ch. 23 - Prob. 1BYBCh. 23 - Prob. 2BYBCh. 23 - Prob. 3BYBCh. 23 - Section 22.1 Why is blood considered to be a...Ch. 23 - Prob. S22.2BYBCh. 23 - Prob. S22.3BYBCh. 23 - Prob. 1TCCh. 23 - Explain why the evolution of the four-chambered...Ch. 23 - Prob. 3TCCh. 23 - Prob. 4TC
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