Pearson eText Biology: Life on Earth with Physiology -- Instant Access (Pearson+)
12th Edition
ISBN: 9780135755785
Author: Gerald Audesirk, Teresa Audesirk
Publisher: PEARSON+
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Chapter 2.3, Problem 1HYEW
The slap of a belly flop provides firsthand experience of the power of cohesion among water molecules. Because of the hydrogen bonds that interconnect its molecules, the water surface resists being broken. When you suddenly force a large number of water molecules apart with your belly, the result can be a bit painful. Do you think that belly-flopping into a pool of (nonpolar) vegetable oil would hurt as much? If you encountered a deep pool filled with vegetable oil, could you float or swim in it?
Why It Hurts So Much to Do a Belly Flop?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 2 Solutions
Pearson eText Biology: Life on Earth with Physiology -- Instant Access (Pearson+)
Ch. 2.1 - What is the mass number of hydrogen? Of helium?Ch. 2.1 - Unstable Atoms Unleashed Because exposure to...Ch. 2.1 - Prob. 2TCCh. 2.1 - What causes the coals of a campfire to glow?Ch. 2.1 - In addition to lower brain activity, what other...Ch. 2.1 - define clement and atom?Ch. 2.1 - Prob. 2CYLCh. 2.1 - explain atomic number and mass number?Ch. 2.1 - explain radioactivity and its dangers and...Ch. 2.1 - describe electron shells?
Ch. 2.2 - At a physical exam, Thomas, a sedentary...Ch. 2.2 - Prob. 1CYLCh. 2.2 - Prob. 2CYLCh. 2.2 - explain atomic number and mass number?Ch. 2.3 - The slap of a belly flop provides firsthand...Ch. 2.3 - Prob. 1TCCh. 2.3 - Predict how a drop of water on an oil-coated...Ch. 2.3 - Prob. 3TCCh. 2.3 - Prob. 1CYLCh. 2.3 - Prob. 2CYLCh. 2.3 - explain atomic number and mass number?Ch. 2.3 - Prob. 1CTCh. 2 - Which of the following is false? a. An dement is...Ch. 2 - Prob. 2MCCh. 2 - Isotopes are defined as a. atoms of the same...Ch. 2 - Prob. 4MCCh. 2 - Prob. 5MCCh. 2 - An atom consists of an atomic nucleus composed of...Ch. 2 - An atom that has lost or gained one or more...Ch. 2 - Prob. 3FIBCh. 2 - Prob. 4FIBCh. 2 - Water is described as __________ because each...Ch. 2 - Prob. 1RQCh. 2 - Distinguish between atoms and molecules and among...Ch. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Detergents help clean by dispersing fats and oils...Ch. 2 - Prob. 2ACCh. 2 - Prob. 3AC
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