Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 23, Problem 14TYPSS
Summary Introduction
To review:
The conclusion drawn from the experiment in which, the G418 molecule, which is a translation inhibitor, has no effect on early stages of cleavage in the fruit fly eggs.
Introduction:
Fertilization of egg cell by the sperm forms zygote. For further development, this zygote undergoes many changes and first one of them is cleavage. Cleavage is the first step towards formation of a multicellular organism. Cleavage is a stage of rapid cell division. During cleavage, the cellular cytoplasm is divided many times but there is no overall growth in the embryo size. These clustered cells are called blastomeres.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 23 Solutions
Biological Science
Ch. 23 - Prob. 1TYKCh. 23 -
2. How is calcium signaling involved in blocking...Ch. 23 - Prob. 3TYKCh. 23 - Prob. 4TYKCh. 23 - Prob. 5TYKCh. 23 - Which of the following does not occur during...Ch. 23 - Prob. 7TYUCh. 23 - Prob. 8TYUCh. 23 - Prob. 9TYUCh. 23 -
10. Plants depend on directional cell expansion...
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