Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 23, Problem 12TYU
Summary Introduction

To review:

The probability that in how many normal fibroblast cells, a complementary deoxyribonucleic acid (cDNA) must be introduced to express at least one MyoD messenger ribonucleic acid (mRNA).

Introduction:

When a somite is formed, a region of it is determined to form the muscle cells. Scientists carried out experiments to know that what causes these cells to commit to their fate and form muscle cells. It was found out by subsequent research that a rare type of mRNA is produced by the proteins of myoblast which makes these somite cells to develop muscle. This rare mRNA was named as MyoD mRNA.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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