SAPLINGPLUS FOR PRINCIPLES OF BIOCHEMIS
SAPLINGPLUS FOR PRINCIPLES OF BIOCHEMIS
7th Edition
ISBN: 9781319424572
Author: nelson
Publisher: MAC HIGHER
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Chapter 22, Problem 8P
Summary Introduction

To determine: The way in which S-adenosylmethionine can be derived from N5-methyltetrahydrofolate, even though the transfer potential of the methyl group in N5-methyltetrahydrofolate is very low.

Introduction:

A cofactor is a “non-protein chemical compound” required for an enzymatic activity. It is also known as "helper molecules" that help in biochemical transformations. For example: S-adenosylmethionine.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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