EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 2.2, Problem 1MQ
- What physical property of cells increases as cells become smaller?
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. Consider a base substitution mutation that occurred in a DNA sequence that resulted in a change in the
encoded protein from the amino acid glutamic acid to aspartic acid. Normally the glutamic acid amino acid
is located on the outside of the soluble protein but not near an active site.
O-H¨
A. What type of mutation occurred?
O-H
B. What 2 types of chemical bonds are found in the R-groups
of each amino acid? The R groups are shaded.
CH2
CH2
CH2
H2N-C-COOH
H2N-C-COOH
1
H
Glutamic acid
H
Aspartic acid
C. What 2 types of bonds could each R-group of each of these amino acids form with other molecules?
D. Consider the chemical properties of the two amino acids and the location of the amino acid in the
protein. Explain what effect this mutation will have on this protein's function and why.
engineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?
Molecular Biology
Please help with question. thank you
You are studying the expression of the lac operon. You have isolated mutants as described below. In the presence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).5. Mutations in the lac operator that strengthen the binding of the lac repressor 200 fold
6. Mutations in the promoter that prevent binding of RNA polymerase
7. Mutations in CRP/CAP protein that prevent binding of cAMP8. Mutations in sigma factor that prevent binding of sigma to core RNA polymerase
Chapter 2 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 2.1 - How do cocci and rods differ in morphology?Ch. 2.1 - Using a microscope, could you differentiate a...Ch. 2.1 - What are the major morphologies of prokaryotic...Ch. 2.2 - What physical property of cells increases as cells...Ch. 2.2 - How can the small size and haploid genome of...Ch. 2.2 - What are the approximate limits to how small a...Ch. 2.2 - How large can a bacterium be? How small? Why is it...Ch. 2.3 - Draw the basic structure of a lipid bilayer and...Ch. 2.3 - Prob. 2MQCh. 2.3 - Prob. 3MQ
Ch. 2.3 - Describe in a single sentence the structure of a...Ch. 2.4 - Why do bacterial cells need cell walls? Do all...Ch. 2.4 - Prob. 2MQCh. 2.4 - What do the enzyme lysozyme and the antibiotic...Ch. 2.4 - Prob. 1CRCh. 2.5 - Prob. 1MQCh. 2.5 - Prob. 2MQCh. 2.5 - Prob. 3MQCh. 2.5 - List several functions of the outer membrane in...Ch. 2.6 - Prob. 1MQCh. 2.6 - Prob. 2MQCh. 2.6 - Prob. 1CRCh. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.7 - Chapter Review How can type IV pili facilitate...Ch. 2.7 - Prob. 1CRCh. 2.8 - Prob. 1MQCh. 2.8 - Chapter Review Why would it be impossible for...Ch. 2.8 - Chapter Review How are magnetosomes and the...Ch. 2.8 - Prob. 1CRCh. 2.9 - Prob. 1MQCh. 2.9 - Prob. 2MQCh. 2.9 - Prob. 1CRCh. 2.10 - Prob. 1MQCh. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.10 - In a few sentences, indicate how the bacterial...Ch. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 1CRCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Contrast the mechanism for motility in...Ch. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.13 - Prob. 3MQCh. 2.13 - Chapter Review How does scotophobotaxis differ...Ch. 2.13 - In a few sentences, explain how a swimming...Ch. 2.14 - Prob. 1MQCh. 2.14 - Prob. 2MQCh. 2.14 - Prob. 3MQCh. 2.14 - List at least three features of eukaryotic cells...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.15 - Prob. 3MQCh. 2.15 - How are the mitochondrion and the hydrogenosome...Ch. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.16 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 3AQ
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- Molecular Biology Please help with question. Thank you You are studying the expression of the lac operon. You have isolated mutants as described below. In the absence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).1. Mutations in the lac repressor gene that would prevent the binding of lactose2. Mutations in the lac repressor gene that would prevent release of lactose once lactose hadbound3. Normally the lac repressor gene is located next to (a few hundred base pairs) and upstreamfrom the lac operon. Mutations in the lac repressor gene that move the lac repressor gene 100,000base pairs downstream.4. Mutations in the lac operator that would prevent binding of lac repressorarrow_forwardYou have returned to college to become a phylogeneticist. One of the first things you wish to do is determine how mammals, birds, and reptiles are related. Like any good scientist, you need to consider all available data objectively and without a preconceived “correct” answer. In pursuit of that, you should produce a phylogenetic tree based only on morphological features that show birds and mammals are more closely related. You will then produce a totally different tree, also using morphological features, that shows birds and reptiles are more closely related. Do not forget to include all three groups in both your trees. Based solely off the trees you produce, which relationship would you consider the more likely and why? Once you have answered that question, provide a brief summary of the “modern” understanding of the relationship between these three groups.arrow_forwardtrue or false, the reason geckos can walk on walls is hydrogen bonding between their foot pads and the moisture on the wall.arrow_forward
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