Three behavioral changes to reduce the risk of heart disease and heart attack. Introduction: Staying active and eating healthy foods is important for the health of the heart.

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Answer 1

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Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

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Answer 3

Question

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

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Answer 4

Question

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

Expert Solution & Answer

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Answer 5

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

Answer 6

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

Answer 7

Chapter 21, Problem 9SA

Summary Introduction

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 21 - Prob. 1SA Ch. 21 - Prob. 2SA Ch. 21 - Prob. 3SA Ch. 21 - Prob. 4SA Ch. 21 - Prob. 5SA Ch. 21 - Prob. 6SA Ch. 21 - Prob. 7SA Ch. 21 - Prob. 8SA Ch. 21 - Prob. 9SA Ch. 21 - Prob. 10SA

Three behavioral changes to reduce the risk of heart disease and heart attack. Introduction: Staying active and eating healthy foods is important for the health of the heart.

Solution Summary: The author enumerates three behavioral changes to reduce the risk of heart disease and heart attack.

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To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.

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Chapter 21 Solutions

Three behavioral changes to reduce the risk of heart disease and heart attack. Introduction: Staying active and eating healthy foods is important for the health of the heart.

Solution Summary: The author enumerates three behavioral changes to reduce the risk of heart disease and heart attack.

Videos

To review: Three behavioral changes to reduce the risk of heart disease and heart attack. Introduction: Staying active and eating healthy foods is important for the health of the heart.

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Chapter 21 Solutions

To review:

Three behavioral changes to reduce the risk of heart disease and heart attack.

Introduction:

Staying active and eating healthy foods is important for the health of the heart.