BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
8th Edition
ISBN: 9781305760738
Author: Campbell
Publisher: CENGAGE C
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Chapter 21, Problem 8RE
REFLECT AND APPLY Why does the degradation of palmitic acid (see Question 12) to eight molecules of acetyl-CoA require seven, rather than eight, rounds of the
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The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S.
b
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The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1.
Calculate the catalytic efficiency with S, T, and U.
Catalytic efficiency with S =
Catalytic efficiency with T =
Catalytic efficiency with U =
Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?
Fumerase catalyzes the conversion of fumerate to malate.
fumerate + H2O ⇋ malate
The turnover number, kcat, for fumerase is 8.00×102 sec-1. The Km of this enzyme for fumerate is 5.00×10-3 μmol mL-1.
a
In an experiment using 2.00×10-3 μmol·mL-1, what is Vmax?
Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.18 M in KH2PO4, you would need 0.25 M Na2HPO4. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO−4]) to equal 0.20 M. What concentration of the Na2HPO4 would be required?
Chapter 21 Solutions
BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
Ch. 21 - REFLECT AND APPLY (a) The major energy storage...Ch. 21 - RECALL What is the difference between...Ch. 21 - RECALL How are lipases activated hormonally?Ch. 21 - RECALL What is the metabolic purpose of linking a...Ch. 21 - RECALL Outline the role of carnitine in the...Ch. 21 - RECALL What is the difference between the type of...Ch. 21 - RECALL Draw a six-carbon saturated fatty acid and...Ch. 21 - REFLECT AND APPLY Why does the degradation of...Ch. 21 - REFLECT AND APPLY Given the nature of the hormonal...Ch. 21 - RECALL Compare the energy yields from the...
Ch. 21 - RECALL Which generates more ATPthe processing of...Ch. 21 - MATHEMATICAL Calculate the ATP yield for the...Ch. 21 - REFLECT AND APPLY It is frequently said that...Ch. 21 - RECALL Describe briefly how -oxidation of an...Ch. 21 - RECALL You hear a fellow student say that the...Ch. 21 - RECALL What are the unique enzymes needed to...Ch. 21 - RECALL What are the unique enzymes needed to...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from the...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from...Ch. 21 - REFLECT AND APPLY How many cycles of -oxidation...Ch. 21 - REFLECT AND APPLY It has been stated many times...Ch. 21 - RECALL Under what conditions are ketone bodies...Ch. 21 - RECALL Briefly outline the reactions involved in...Ch. 21 - REFLECT AND APPLY Why might a doctor smell the...Ch. 21 - REFLECT AND APPLY Why might a person who is an...Ch. 21 - REFLECT AND APPLY A friend who is trying to lose...Ch. 21 - RECALL Compare and contrast the pathways of fatty...Ch. 21 - RECALL Outline the steps involved in the...Ch. 21 - RECALL What is the metabolic importance of...Ch. 21 - RECALL In fatty acid degradation, we encounter...Ch. 21 - RECALL How are the two redox reactions of...Ch. 21 - RECALL How is ACP similar to coenzyme A? How is it...Ch. 21 - RECALL What is the purpose of having ACP as a...Ch. 21 - RECALL Why are linoleate and linolenate considered...Ch. 21 - REFLECT AND APPLY Is it possible to convert fatty...Ch. 21 - REFLECT AND APPLY What is the role of citrate in...Ch. 21 - REFLECT AND APPLY In the mitochondrion is a...Ch. 21 - REFLECT AND APPLY In fatty acid synthesis,...Ch. 21 - REFLECT AND APPLY (a) Where in an earlier chapter...Ch. 21 - RECALL What is the source of the glycerol in...Ch. 21 - RECALL What is the activating group used in the...Ch. 21 - RECALL What are the differences between synthesis...Ch. 21 - RECALL How are isoprene units important in...Ch. 21 - RECALL A cholesterol sample is prepared using...Ch. 21 - Prob. 46RECh. 21 - Prob. 47RECh. 21 - Prob. 48RECh. 21 - Prob. 49RECh. 21 - REFLECT AND APPLY A drug that reduces blood...Ch. 21 - RECALL What role does neuropeptide Y play in...Ch. 21 - Prob. 52RECh. 21 - Prob. 53RECh. 21 - Prob. 54RECh. 21 - RECALL What is the connection between insulin and...
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