(a) Interpretation: The reason the proteins are not visible in the lanes without amylase treatment should be discussed. Concept introduction: Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules. SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 1

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S. b Question Content Area The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1. Calculate the catalytic efficiency with S, T, and U. Catalytic efficiency with S = Catalytic efficiency with T = Catalytic efficiency with U = Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?

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Answer 2

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 3

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 4

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 5

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 6

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 7

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 12

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Answer 13

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Answer 15

Ch. 21 - Prob. 1P Ch. 21 - Prob. 2P Ch. 21 - Prob. 3P Ch. 21 - Prob. 4P Ch. 21 - Prob. 5P Ch. 21 - Prob. 6P Ch. 21 - Prob. 7P Ch. 21 - Prob. 8P Ch. 21 - Prob. 9P Ch. 21 - Prob. 10P

Solution Summary: The author explains that the proteins are not visible in the lanes without amylase treatment.

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