Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Chapter 2.1, Problem 3EQ
Summary Introduction
To describe: The interpretation of the data “98% of particles passed through the gold foil.”
Introduction: Atom is the smallest structural unit of any matter. Combination of a great number of atoms results in the origination of a substance. The living organisms are also originated from the combination of atoms. A group of atoms joined together and forms the molecule.
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(b) Table Q1 below provides optical measurements of samples with known concentrations of
analyte (ten samples were measured and the average and standard deviation are presented).
They are also represented graphically in Figure Q1, below the table.
Analyte
concentration
(nM)
0.1
0.2
0.5
1
2
5
10
15
fluorescence intensity
300000
250000
200000
150000
100000
50000
0
0
Table Q1
Fluorescence
intensity
(average)
9300
9200
10800
19600
37200
90000
178000
224000
5
[Analyte]/nM
10
Standard
deviation
2000
1200
1000
2000
10000
30000
25000
40000
15
Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard
deviation.
(ii) Calculate the limit of detection (LOD) for this sensor.
(iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a
signal of 142800. The coefficient of variation of the device is 5%. Explain whether the
diagnostic lab would have the confidence to report that the sample has a concentration
in analyte of 8 nM or…
* You can keep extra decimal places through most of this problem, but round your
final estimate to one decimal place*
You wish to quantify your favourite compound in an experimental solution using
spectrophotometry. First, you use standard solutions of your favourite compound to
collect the following standard curve data. Calculate the slope of the line of best fit.
Standard curve data
Conc (mM)
0.300
0.600
0.900
1.200
1.500
Abs
0.150
0.300
0.450
0.600
0.750
Next, you prepare an experimental sample by mixing 0.50 mL of the compound
solution with 9.50 mL of distilled water, and you read an absorbance of 0.625 for this
sample. Calculate the concentration (mM) of the original solution of compound.
Round this answer to one decimal place.
Which is the best method for producing phase-separated GUVs (at least 20 um in size)?
Chapter 2 Solutions
Biology
Ch. 2.1 - Before the experiment conducted by Ernest...Ch. 2.1 - What was the hypothesis tested by Rutherford?Ch. 2.1 - Prob. 3EQCh. 2.1 - Explain the difference between an electron shell...Ch. 2.2 - Prob. 1CCCh. 2.2 - Prob. 2CCCh. 2.3 - When oil dissolves in a soapy solution, where is...Ch. 2.3 - Prob. 2CCCh. 2.3 - Core Skill: Connections Look ahead to Figure...Ch. 2 - _______________ make(s) up the nucleus of an atom....
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