Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 21, Problem 21.3CC

(1)

Summary Introduction

To compare: To compare and contrast Northern blot versus Western blot

Introduction: The blotting techniques are used to separate different macromolecules on the basis of size and mass or charge on the macromolecule. These blotting techniques have enabled the separation of protein, RNA and DNA molecules according to their charge and mass. The segregation of the similar sized fragments has revolutionized the biotechnology, biochemistry, genomics, proteomics, and associated life sciences domain. The development of vaccines, drugs, insect resistant plants, and fortified foods are the result of the study of genes with the help of blotting techniques.

Summary Introduction

To compare: To compare and contrast yeast two-hybrid assay with coimmunoprecipitation.

Introduction: There are a number of protein-protein interactions occurring inside the cell. These interactions play an important role in post-translational modifications, activation of enzymes, changing the configuration of proteins and start of biochemical reactions. The conditions in which protein-protein interaction takes place tells us about how and when a protein is being translated from RNA and gives the frequency of the release of the protein.

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