The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes. Introduction: To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb? Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

Design a grafting experiment to determine if limb mesoderm determines forelimb / hindlimb identity. Include the experiment, a control, and an interpretation in your answer.

The Snapdragon is a popular garden flower that comes in a variety of colours, including red, yellow, and orange. The genotypes and associated phenotypes for some of these flowers are as follows: aabb: yellow AABB, AABb, AaBb, and AaBB: red AAbb and Aabb: orange aaBB: yellow aaBb: ? Based on this information, what would the phenotype of a Snapdragon with the genotype aaBb be and why? Question 21 options: orange because A is epistatic to B yellow because A is epistatic to B red because B is epistatic to A orange because B is epistatic to A red because A is epistatic to B yellow because B is epistatic to A

Answer 2

Question

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Answer 6

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Answer 7

Chapter 21, Problem 1RQ

Summary Introduction

To review:

The method to analyse the metagenome of a microbial community and the advantages and disadvantages of metagenomics as compared to culturing microbes.

Introduction:

To identify and study microorganisms, the microbes need to be cultured on nutrient media. Most of the microbes are difficult to culture and hence, cannot be characterized completely. Norman Pace was the first one to identify the microbe, by sequencing its Deoxyribonucleic acid (DNA). Metagenomics deals with the sequencing of the entire DNA extracted from the microbial community.

Answer 8

Expert Solution & Answer

Explanation of Solution

The sequencing and analysis of the metagenome require a series of steps. The DNA samples are collected from the target community after separating the targeted microbes from their natural environment. The DNA is then isolated and purified from the microbes of interest. The small-subunit ribosomal RNA (SSU rRNA) amplification is done to study the species diversity.

The extracted and purified DNA can be cloned and sequenced. The assembling of sequenced DNA into genome can also reveal the diversity of species. One such technique is Illumina, which generates a large amount of data and require computational analysis. The sequenced DNA fragments are assembled using mathematical tools. The ecological functions of the microbes can be studied by functional annotations.

The limitations of the metagenomics are: it is difficult to assign the correct DNA sequence to the shared genomes. Different computational pipelines may predict different partial genomes. It is difficult to discover genomes of close relatives using pipelines based on reference genomes.

The metagenomics is an important toll that may reveal the characteristics and species diversity of an organism that cannot be cultured. It can also play a vital role in determining the species of microbes that are related closely. The evolutionary line of a microbe can also be revealed by the metagenomics.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Ch. 21.1 - Prob. 1TQ Ch. 21.1 - Prob. 2TQ Ch. 21.1 - Prob. 3TQ Ch. 21.2 - Prob. 1TQ Ch. 21.4 - Prob. 1TQ Ch. 21.4 - Prob. 2TQ Ch. 21.4 - Prob. 3TQ Ch. 21.5 - Prob. 1TQ Ch. 21.6 - Prob. 1TQ Ch. 21.6 - Prob. 2TQ

Concept explainers

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 21 Solutions