CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 2.1, Problem 1MI
Summary Introduction
The focal length of a system is the distance between the center of the curved mirror or a lens and its focus. In microscopy, this term refers to the amount of distance which is required between the top surface of the object or sample and the objective lens, so as to view the image through the microscope that is in-focus.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 2 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1CCCh. 2.1 - Prob. 2CCCh. 2.1 - Prob. 3CCCh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1CCCh. 2.2 - Prob. 2CCCh. 2.2 - Prob. 3CC
Ch. 2.2 - Prob. 4CCCh. 2.2 - Prob. 5CCCh. 2.2 - Prob. 6CCCh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1CCCh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Prob. 3CCCh. 2.3 - Prob. 4CCCh. 2.4 - Prob. 1MICh. 2.4 - Prob. 1CCCh. 2.4 - Prob. 2CCCh. 2.4 - Prob. 3CCCh. 2.4 - Prob. 4CCCh. 2.5 - Prob. 1MICh. 2.5 - Prob. 1CCCh. 2.5 - Prob. 2CCCh. 2 - Prob. 1RCCh. 2 - Prob. 2RCCh. 2 - Prob. 3RCCh. 2 - Prob. 1ALCh. 2 - Prob. 2ALCh. 2 - Prob. 4AL
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