CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 2, Problem 2AL
Summary Introduction
A photograph or a digital image that is taken with the help of a microscope is known as a micrograph or photomicrograph. A magnified image of the specimen is obtained from the micrograph. Micrography can be defined as the art or practice of using a light microscope to make photographs. It provides wide-spread details of the microstructure of the specimens.
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What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
Chapter 2 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1CCCh. 2.1 - Prob. 2CCCh. 2.1 - Prob. 3CCCh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1CCCh. 2.2 - Prob. 2CCCh. 2.2 - Prob. 3CC
Ch. 2.2 - Prob. 4CCCh. 2.2 - Prob. 5CCCh. 2.2 - Prob. 6CCCh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1CCCh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Prob. 3CCCh. 2.3 - Prob. 4CCCh. 2.4 - Prob. 1MICh. 2.4 - Prob. 1CCCh. 2.4 - Prob. 2CCCh. 2.4 - Prob. 3CCCh. 2.4 - Prob. 4CCCh. 2.5 - Prob. 1MICh. 2.5 - Prob. 1CCCh. 2.5 - Prob. 2CCCh. 2 - Prob. 1RCCh. 2 - Prob. 2RCCh. 2 - Prob. 3RCCh. 2 - Prob. 1ALCh. 2 - Prob. 2ALCh. 2 - Prob. 4AL
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- The diffraction limit is an unbeatable barrier limiting the effective resolution of light microscopy due to the inherent wavelength of light.True or Falsearrow_forwardPlease provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY? Please answer in detail; thank youarrow_forwardWhat factors must be addressed to properly acquire quantitative fluorescence microscopy images? What information about the imaging system should be recorded? What controls are needed and what standards are available?arrow_forward
- What do you mean by deconvolution microscopy technique?arrow_forwardPROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observationarrow_forwardcan you please answer these in orderarrow_forward
- Calculate the width of all 3 cells in micrometers. Use scale. Of a 40x objective.arrow_forwardState the function of each part of the compound light microscope.arrow_forwardIf magnification of the two image-forming lenses are both 10X, the total magnification of the image will be 1X 10X 100X 1000X Question 9 (1 point) The distance through which a microscopic specimen can be moved and still have it remain in focus is called the magnification working distance depth of field field of view Question 10 (1 point) What adjustment do you use to focus a 40X objective? Coarse focus Fine focus Botharrow_forward
- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forwardWhen you are trying to find a specimen, you should use; The scanning objective The low/medium power objective The high power objectivearrow_forwardComplete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30arrow_forward
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