ANATOMY AND PHYSIOLOGY ONLINE MATERIALS,
4th Edition
ISBN: 9781265761493
Author: McKinley
Publisher: MCG
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Chapter 20.3, Problem 10WDYL
Which two pressures have the largest values? Explain how each of these specifically influences filtration and reabsorption.
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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 20 Solutions
ANATOMY AND PHYSIOLOGY ONLINE MATERIALS,
Ch. 20.1 - What are three differences in anatomic structure...Ch. 20.1 - Prob. 2WDYLCh. 20.1 - What type of capillary is the most permeable, and...Ch. 20.1 - Prob. 4WDYLCh. 20.1 - Prob. 5WDYLCh. 20.2 - In which type of vessel is blood flow the slowest?...Ch. 20.3 - What substances are transported by diffusion...Ch. 20.3 - Prob. 8WDYLCh. 20.3 - How does the hydrostatic pressure change from the...Ch. 20.3 - Which two pressures have the largest values?...
Ch. 20.3 - If these lymph vessels were nonfunctional, what...Ch. 20.4 - In what ways is angiogenesis stimulated in...Ch. 20.4 - Prob. 13WDYLCh. 20.4 - What relationship exists between metabolic...Ch. 20.4 - Prob. 15WDYLCh. 20.5 - Prob. 16WDYLCh. 20.5 - Prob. 17WDYLCh. 20.5 - How is the small pressure gradient in veins...Ch. 20.5 - How is the pressure gradient to move blood through...Ch. 20.5 - How is resistance defined?Ch. 20.5 - What are the three factors that alter resistance?...Ch. 20.5 - Prob. 22WDYLCh. 20.6 - Prob. 23WDYLCh. 20.6 - What is the initial change to blood pressure when...Ch. 20.6 - Prob. 25WDYLCh. 20.6 - Prob. 26WDYLCh. 20.7 - Which organs have an increased proportion of...Ch. 20.8 - Prob. 28WDYLCh. 20.8 - Prob. 29WDYLCh. 20.9 - Prob. 30WDYLCh. 20.9 - Prob. 31WDYLCh. 20.10 - Prob. 32WDYLCh. 20.10 - Prob. 33WDYLCh. 20.10 - Prob. 34WDYLCh. 20.10 - What are the systemic arteries that supply...Ch. 20.10 - Prob. 36WDYLCh. 20.10 - Prob. 37WDYLCh. 20.10 - Prob. 38WDYLCh. 20.11 - Prob. 39WDYLCh. 20.11 - Prob. 40WDYLCh. 20.11 - Prob. 41WDYLCh. 20.11 - Prob. 42WDYLCh. 20.12 - List the five structures of fetal circulation, and...Ch. 20.12 - Prob. 44WDYLCh. 20 - _____ 1. Which of the following is not a...Ch. 20 - _____ 2. Which statement is accurate about veins?...Ch. 20 - _____ 3. Vasa vasorum are found in the tunica...Ch. 20 - _____ 4. Which of the following decreases...Ch. 20 - _____ 5. The __________ is a type of vessel with...Ch. 20 - _____ 6. An increase in _____ will result in an...Ch. 20 - Prob. 7DYKBCh. 20 - _____ 8. Velocity of blood flow is the slowest in...Ch. 20 - _____ 9. Blood pressure is regulated by the a....Ch. 20 - _____ 10. Name the correct pathway that blood...Ch. 20 - Prob. 11DYKBCh. 20 - Prob. 12DYKBCh. 20 - Explain the difference between hydrostatic and...Ch. 20 - Write the formula for determining net filtration...Ch. 20 - Prob. 15DYKBCh. 20 - Prob. 16DYKBCh. 20 - Briefly explain how changes in cardiac output,...Ch. 20 - Compare how the cardiac center and vasomotor...Ch. 20 - Prob. 19DYKBCh. 20 - What postnatal changes occur in the heart and...Ch. 20 - If a patient has cirrhosis of the liver and is...Ch. 20 - Prob. 2CALCh. 20 - Prob. 3CALCh. 20 - Prob. 4CALCh. 20 - Prob. 5CALCh. 20 - Prob. 1CSLCh. 20 - Arteries tend to have a lot of vascular...Ch. 20 - Explain why an overweight individual with high...
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- 1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forward1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
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