Genetics: A Conceptual Approach
Genetics: A Conceptual Approach
6th Edition
ISBN: 9781319127121
Author: Pierce
Publisher: MAC HIGHER
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Chapter 20.2, Problem 34AQP
Summary Introduction

To explain:

The conclusions can be drawn about the genes that might be implicated in antibiotic resistance in these bacteria.

Introduction:

Antibiotic resistance causes growth of various strains of bacteria even after the addition of antibiotics. These strains secrete an enzyme beta-lactamase which degrades the antibiotic ring causing growth of bacteria in the culture media.

Summary Introduction

To explain:

How might this information be useful to design new antibiotics that are less vulnerable to resistance.

Introduction:

Antibiotics are the drugs which kill various strains of bacteria. These drugs inhibit the formation of peptidoglycan layer in bacteria or prevent the synthesis of ribosomal proteins.

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Draw a rough sketch of the control and experimental data using a normal dose/response curve plot: i.e. % of total bound ligand vs. concentration. Indicate Kd and Bmax on this sketch. You don’t need to use exact numbers, but the relative proportions should be close
Name something that could be happening to glutamate transporters that could have this effect on glutamate transport (i.e. the difference in glutamate transporters between control and experimental conditions). (Bonus: if you can also guess what the experimental condition is).
a. For the control condition, calculate Kd. Include units and show your work. b. For the control condition, calculate Bmax. Include units and show your work.  c. For the experimental condition, calculate Kd. Include units and show your work. For the experimental condition, calculate Bmax. Include units and show your work.
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