Chapter 20, Problem 9TYU

Summary Introduction

To explain: The cloning of the human crystallin gene to obtain a sufficient amount of the human crystallin protein.

Concept introduction:

Protein encoding genes express themselves and form functional gene products in the form of proteins. This is known as gene expression. Generally, polymerase chain reaction (PCR) is used for the amplification of the gene sequence of interest and to synthesize its multiple copies. This gene fragment is then inserted into the expression vector to synthesize the protein encoded by the gene.