Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
10th Edition
ISBN: 9780133922851
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 20, Problem 1TYU

In DNA technology, the term vector can refer to

  • (A) the enzyme that cuts DNA into restriction fragments.
  • (B) the sticky end of a DNA fragment.
  • (C) a SNP marker.
  • (D) a plasmid used to transfer DNA into a living cell.
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Why is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple copies of itself. b.) It joins two DNA fragments together. c.) It shapes bacterial DNA into a circular plasmid. d.) It cuts DNA into restriction fragments.
Which of the following tools of DNA technology is incorrectlypaired with its use?(A) electrophoresis—separation of DNA fragments(B) DNA ligase—cutting DNA, creating sticky ends of restriction fragments(C) DNA polymerase—polymerase chain reaction to amplifysections of DNA(D) reverse transcriptase—production of cDNA frommRNA
How are "sticky ends" generated in DNA? A) by gel electrophoresis B) by cutting with restriction enzymes C) by using DNA polymerase D) by adding DNA ligase E) both A and B are correct
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