Nester's Microbiology: A Human Perspective
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 20, Problem 9MC
Summary Introduction

Introduction:

Antiviral medications are the drugs that are used to treat viral infections. Acyclovir is an antiviral drug, which is used to treat herpes virus infection. Herpes is caused by HSV (herpes simplex virus). It affects thegenital region, mucous surfaces, and anal regions. It is usually a long-term infection and its symptoms are hardly visible. It is a sexually transmitted disease. Commonly visible symptoms include the appearance of blisters on external genitalia, pain, itching, fever, and cold sores.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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