Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 20, Problem 8P
Interpretation Introduction

(a)

Interpretation:

The radioactive compound obtained in five seconds after algae was exposed to radioactive carbon dioxide should be identified.

Concept introduction:

The consecutive steps of the Calvin cycle should be analyzed to identify the compound contained radioactivity after five seconds.

Interpretation Introduction

(b)

Interpretation:

The radioactive compound obtained after sixty seconds exposure to algae to radioactive carbon dioxide should be identified.

Concept introduction:

The consecutive steps of the Calvin cycle were analyzed to identify the compounds containing radioactivity after sixty seconds.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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