Campbell Biology Custom Stony Brook 10 Th Edition
Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Chapter 20, Problem 7TYU

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problm of

  • (A) post -transcriptional processing.
  • (B) post-translational processing.
  • (C) nucleic acid hybridization.
  • (D) restriction fragment ligation.
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Codon optimization is a widely used process for recombinant expression in prokaryotic systems. Regarding this process, mark the incorrect alternative. * A)increases the overall yield of expression of recombinant proteins. B)prevents the formation of truncated proteins. C)increases the frequency of tRNAs that recognize rare codons. D)does not interfere with the primary sequence of the recombinant protein.
Which of the following is not true of cDNA produced using human brain tissue as the starting material? (A) It can be amplified by the polymerase chain reaction. (B) It was produced from pre-mRNA using reverse transcriptase. (C) It can be labeled and used as a probe to detect genes expressed in the brain. (D) It lacks the introns of the premRNA
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