BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
9th Edition
ISBN: 9781319425784
Author: BERG
Publisher: Macmillan Higher Education
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Chapter 20, Problem 43P
Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-1M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, KM is the substrate concentration at half of the maximum velocity. The smaller the KM of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

kcat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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