Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 3CT
You have isolated a bacterium that produces an antibiotic. The bacterium grows well at 37°C but doesn’t produce the antibiotic at that temperature. Antibiotic production is best at 30°C, but the cells grow so poorly at that temperature that you can’t get enough antibiotic. The relationships are shown in the following graphs. Design a procedure to maximize the biomass (total cells) and the yield of the antibiotic.
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Solve the following problem:
34,000 bacteria/l have been measured 4 hours after inoculating a medium. After 24 hours, the number of bacteria increased to 5.2x10^6/l. Assuming that the lag phase is negligible, calculate:
The specific speed of growth.
The number of bacteria that were inoculated.
A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism.
a) What is the time required to produce 15 g of biomass?
!!!!! ANS IS 3.07 HRS!!!!
STEPS on how to reach this please
Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.
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Laboratory Experiments in Microbiology (11th Edition)
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