Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 2CT
Summary Introduction
To determine:
The method for obtaining the desired cell concentration form the 24-hour flask along with plotting graph of data given.
Introduction:
Microbial culture or
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The number of bacterial cells in a culture broth is to be determined by a culture
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Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with
warm molten agar and poured into a Petri dish. The numbers of bacterial colony
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What is the number of colony forming units per mL of the culture broth? Choose only
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-3
Plate 1 10 dilution
Plate 2 10-4 dilution
Plate 3 10-5 dilution
Plate 4 10 dilution
Plate 5 10 dilution
-8
Plate 6 100 dilution
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Number of colony forming units (CFU)
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1
0
m
The number of bacterial cells in a culture broth is to be determined by a culture
technique.
Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with
warm molten agar and poured into a Petri dish. The numbers of bacterial colony
forming units (CFU) after overnight incubation are shown in the table below.
What is the number of colony forming units per mL of the culture broth? Choose only
the most appropriate plate for your calculation. Give your answer as the number only
(do not add text for the units). You may use scientific notation with the format
1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a
whole number.)
Plate Dilution
Plate 1 10 dilution
Plate 2 10 dilution
-5
Plate 3 10 dilution
Plate 4 10 dilution
Plate 5 107 dilution
-8
Plate 6 100 dilution
*Too many to count
Number of colony forming units (CFU)
TMTC*
TMTC*
TMTC*
867
154
18
Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?
Chapter 20 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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