(a) Interpretation: The values of k c a t C O 2 / K M C O 2 and k c a t O 2 / K M O 2 as s- 1 M-1 should be determined. Concept introduction: If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further. The Michael is constant, K M is the substrate concentration at half of the maximum velocity. The smaller the K M of an enzyme, the smaller the amount of substrate required to saturate the enzyme. k cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

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Answer 1

Question

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)

a) What are the differences between the Direct & Indirect Immunofluorescence Assays? b) What are the advantages of the Indirect Immunofluorescence Assays? c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

Answer 2

Question

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Answer 3

Question

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Expert Solution & Answer

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Answer 5

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 6

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Answer 7

Chapter 20, Problem 36P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 20 - Prob. 1P Ch. 20 - Prob. 2P Ch. 20 - Prob. 3P Ch. 20 - Prob. 4P Ch. 20 - Prob. 5P Ch. 20 - Prob. 6P Ch. 20 - Prob. 7P Ch. 20 - Prob. 8P Ch. 20 - Prob. 9P Ch. 20 - Prob. 10P

Solution Summary: The author explains how to calculate the ratios by substituting the values of k_catCO

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(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Chapter 20 Solutions

Solution Summary: The author explains how to calculate the ratios by substituting the values of k_catCO

Concept explainers

Videos

(b) Interpretation: The reason for the occurrence of oxygenation reaction should be determined. Concept introduction: The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Chapter 20 Solutions