EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 20, Problem 30ESP
A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the melting temperature, Tm(°C), which is computed by the equation 81.5 + 0.41 × (%GC) – (675/N), where %GC is the percentage of GC
5′-TTGAAAATATTTCCCATTGCC-3′
compute the annealing temperature for PCR. What is the relationship between Tm (°C) and %GC? Why? (Note: In reality, this computation provides only a starting point for empirical determination of the most useful annealing temperature.)
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calculate the questions showing the solution including variables,unit and equations all the questiosn below using the data
a) B1,
b) B2,
c) hybrid rate constant (1)
d) hybrid rate constant (2)
e) t1/2,dist
f) t1/2,elim
g) k10
h) k12
i) k21
j) initial concentration (C0)
k) central compartment volume (V1)
l) steady-state volume (Vss)
m) clearance (CL) AUC (0→10 min) using trapezoidal rule
n) AUC (20→30 min) using trapezoidal rule
o) AUCtail (AUC360→∞)
p) total AUC (using short cut method)
q) volume from AUC (VAUC)
Chapter 20 Solutions
EBK CONCEPTS OF GENETICS
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