MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
3rd Edition
ISBN: 2810022612789
Author: Cowan
Publisher: MCG
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Chapter 2, Problem 9Q
Summary Introduction
To determine:
The history of the discovery of penicillin by expanding the story given
Concept introduction:
Penicillin is an antibiotic which is used to treat various bacterial infections. It is the first discovered antibiotic which is obtained naturally. It is an antibiotic which is capable of competing, inhibiting, or killing several microbial species.
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)What methods are similar in bringing fungal and bacterial specimens from a particular body site?Note that these questions are not about what happens to the specimens in the lab. It is about taking specimens from the human body.
2)What are some methods that might be more suitable for bringing fungal pathogens to the lab?
3)What methods might be more suitable for bacteria?
Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 1 x 10 fungal
CFU/mL (or CFU/g) the entire lot produced will be rejected. Kousei's boss, Kaori, wanted to know if the lot passed
the FDA guidelines. The following fungal counts were obtained after serial dilution and spread plating of the
samples:
Type of
Dilutions plated
10-4
10-3
105
Colony
Plate 1
Plate 2
Plate 1
Plate 2
Plate 1
Plate 2
Plate 3
166
Fungi
160
155
45
40
1
11
Based on the fungal counts obtained, will the lot be rejected?
No, because the computed CFU/mL is 4.3 x 10^5
No, because the computed CFU/mL is 4.1 x 10^5
Yes, because the computed CFU/mL is 4.3 x 10^6
Yes, because the computed CFU/mL is 4.1 x 10^6
Yes, because the computed CFU/mL is 1.1 X 10^7
Information is lacking so CFU/mL cannot be computed
Plate 3
37
Plate 3
7
W
S
Fungal infections generally have fewer treatment options because..
a) they are Gram negative cells
b) their cell type is eukaryotic like ours
c) they can easily mutate their genetic code
d) their DNA is more complex than ours
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Chapter 2 Solutions
MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
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- In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only contained antibiotics but also inhibitors of other processes such as cholesterol metabolism. Over a period of several years, he screened more than 6,000 fungal cultures until a positive result emerged. This compound, which was named compactin, came from the blue-green mold Penicillium citrinum Pen-5 that was isolated from a rice sample collected at a grain shop in Kyoto. Based on biochemical assays, compactin is the first known statin. 1. The original kinetics data for compactin inhibition of HMG-CoA reductase enzyme that was published by Dr. Endo in 1976 is shown on the right. a) Determine the Km and Vmax values for enzymatic activity. Do NOT forget the units. Answers can be expressed either as a fraction or in decimals. With No inhibitor: ; Vmax= -60 1/V, (nanomoles/min)-1 0.4 0.35+ 0.3 0.25 0.2 0.15 0.1 0.05 -40 -20 0 + 24 nM compactin + 70 nM compactin None T 20 40 60 1/[HMG-CoA], (mM)-1…arrow_forwardAn apple farmer notices that some of the trees in his orchard have a fungus on them and those trees have necrotic ring spots typically associated with a viral infection. The trees that don't have fungus appear to be healthy. What is the most likely conclusion the farmer can make here?arrow_forwardHistorians report that 2500 years ago the Chinese learned to treat superficial infections such as boils by applying moldy soybean curds to the area. Can you suggest what this implies? - Please dont use the same answer that is already posted.arrow_forward
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