MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
3rd Edition
ISBN: 2810022612789
Author: Cowan
Publisher: MCG
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Chapter 2, Problem 20Q
Summary Introduction
To explain:
The difference in the results when a meter is multiplied with
Concept introduction:
Metric system is also called as the decimal system of measurement. Now, it is known as SI unit (International System of Unit). Scientists have adopted the metric system as the most common system of measurement of lengths and weights.
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You wish to quantify your favourite compound in an experimental solution using
spectrophotometry. First, you use standard solutions of your favourite compound to
collect the following standard curve data. Calculate the slope of the line of best fit
to two decimal places, and enter it in the box below the table.
Standard curve data
Conc (MM)
0.300
0.600
0.900
1.200
1.500
Abs
0.225
0.450
0.675
0.900
1.125
Next, you prepare an experimental sample by mixing 0.25 mL of the compound
solution with 9.75 mL of distilled water. Calculate the dilution factor and enter it as a
whole number (no decimals).
Next, you read an absorbance of 0.72 absorbance units for the diluted sample.
Calculate the concentration (mm) of the original solution of the compound. Round
this answer to one decimal place.
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It is possible to make a perfectly precise measurement?Explain.
You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. For
determination of Fe concentration in the flour sample, you conducted an atomic absorption
spectrometer (AAS) experiment using known concentration of Fe (standard). About 10 g of
flour sample is used and mixed in 100 mL of solution then measured using AAS.
1.2
0.8
0.6
0.4
0.2
8
10
12
-0.2
Fe concentration (mg/L)
[a] If the absorbance value of the flour sample is at 0.35, calculate the Fe concentration
presence in the flour sample (obtained equation from the standard curve is y = 0.1018x -
0.0514).
[b] Commonly, the Fe contents are in the range of 1.6 mg to 2.3 mg in 100 g super flour to
comply with FDA's standard of identity. Based on your calculation, justify if the Fe
concentration in the flour sample comply with the FDA specification.
Absorbance at 248.3 nm
Chapter 2 Solutions
MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
Ch. 2.1 - Explain what the Five Is are and what each step...Ch. 2.1 - Discuss three physical states of media and when...Ch. 2.1 - Compare and contrast selective and differential...Ch. 2.1 - Provide brief definitions for defined media and...Ch. 2.1 - Medical Moment The Making of the Flu Vaccine: An...Ch. 2.1 - Prob. 1NPCh. 2.1 - Prob. 2NPCh. 2.2 - Prob. 5AYPCh. 2.2 - Prob. 6AYPCh. 2.2 - Prob. 7AYP
Ch. 2.2 - Give examples of simple, differential, and special...Ch. 2.2 - Prob. 3NPCh. 2.2 - Medical Moment Gram-Positive Versus Gram-Negative...Ch. 2 - The identities of microorganisms on our planet a....Ch. 2 - Prob. 2QCh. 2 - Often bacteria that are freshly isolated from a...Ch. 2 - Which of these types of organisms is least likely...Ch. 2 - Prob. 5QCh. 2 - Some bacteria can produce a structure called an...Ch. 2 - A fastidious organism must be grown on what type...Ch. 2 - Write a short paragraph to differentiate among the...Ch. 2 - Prob. 9QCh. 2 - Viruses are commonly grown in/on a. animal cells...Ch. 2 - Can you devise a growth medium with ingredients...Ch. 2 - There is a type of differential medium that can...Ch. 2 - Prob. 13QCh. 2 - Several bacteria live naturally in a material on...Ch. 2 - Archaea often grow naturally in extreme...Ch. 2 - Prob. 16QCh. 2 - After performing the streak plate procedure on a...Ch. 2 - You are a scientist studying a marsh area...Ch. 2 - Prob. 19QCh. 2 - Prob. 20QCh. 2 - You perform the special stain for bacterial...Ch. 2 - Prob. 1VC
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