EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 2, Problem 7FIB
Fill in the Blanks
7. Reactions that release energy are called ______________ reactions.
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CONTENTS OF REACTION CUVETTES: (Calculations in PreLab)
[ONPG]
Diluted ONPG
0mM
None
0.25mL
0.5mL
0.75mL
1.0mL bloo
1.25mL
1.5mL
Buffer
Lactase
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
0.25mL
*** HINT: To calculate [ONPG], cuvette #5 contains 1.0mL ONPG in a total of 2.0mL. (This is the same as a 1:2
dilution.) Therefore we divide the concentration of ONPG by 2 to get 5mM.
5mM
Water
1.5mL
1.25mL
1.0mL
0.75mL
0.5mL
0.25mL
None
***
4. To perform Lactase Assay with first cuvette ("OmM"):
Clean cuvette with Kimwipe.
Place cuvette into Spectro Vis with stripes in front in back.
GET GROUP READY FOR TIMED ASSAY. AT THE SAME TIME:
* Press Record (Green Triangle button).
* Add 0.25mL of Lactase to cuvette. Use pipette to quickly mix liquid.
WAIT 60 SECONDS! After the assay is complete:
* In Experiment menu, select "Store Latest Run" THIS STEP IS NECESSARY!
* Record data on the data table below.
5. Repeat Step 7 for all of your cuvettes. Record…
Chapter 2 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 2 - Electrons zip around the nucleus at about 5...Ch. 2 - Chlorine and potassium atoms form ionic bonds,...Ch. 2 - Why are decomposition reactions exothermic, that...Ch. 2 - Why does the neutralization of an acid by a base...Ch. 2 - Raw Oysters and Antacids: A Deadly Mix? The highly...Ch. 2 - Why do the cell membranes of microbes living in...Ch. 2 - Prob. 1MCCh. 2 - The atomic mass of an atom most closely...Ch. 2 - One isotope of iodine differs from another in...Ch. 2 - Prob. 4MC
Ch. 2 - Which of the following terms most correctly...Ch. 2 - In water, cations and anions of salts dissociate...Ch. 2 - Prob. 7MCCh. 2 - Which of the following statements about a...Ch. 2 - Proteins are polymers of ___________. a. amino...Ch. 2 - Which of the following are hydrophobic organic...Ch. 2 - Fill in the Blanks 1. The outermost electron shell...Ch. 2 - Fill in the Blanks 2. The type of chemical bond...Ch. 2 - Prob. 3FIBCh. 2 - Prob. 4FIBCh. 2 - Fill in the Blanks 5. Groups of atoms such as NH2...Ch. 2 - Fill in the Blanks 6. The reverse of dehydration...Ch. 2 - Fill in the Blanks 7. Reactions that release...Ch. 2 - Fill in the Blanks 8. All chemical reactions begin...Ch. 2 - Fill in the Blanks 9. The ____________ scale is a...Ch. 2 - Prob. 10FIBCh. 2 - Label a portion of the molecule below; label two...Ch. 2 - Shown is the amino acid tryptophan. Put the letter...Ch. 2 - List three main types of chemical bonds, and give...Ch. 2 - Name five properties of water that are vital to...Ch. 2 - Prob. 3SACh. 2 - What is the difference between atomic oxygen and...Ch. 2 - Explain how the polarity of water molecules makes...Ch. 2 - Prob. 1CTCh. 2 - Prob. 2CTCh. 2 - Two freshmen disagree about an aspect of...Ch. 2 - When an egg white is heated, it changes from...Ch. 2 - Prob. 5CTCh. 2 - The poison glands of many bees and wasps contain...Ch. 2 - Prob. 7CTCh. 2 - Prob. 8CTCh. 2 - The deadly poison hydrogen cyanide has the...Ch. 2 - Triple covalent bonds are stronger and more...Ch. 2 - How can hydrogen bonding between water molecules...Ch. 2 - How can a single molecule of magnesium hydroxide...Ch. 2 - Prob. 13CTCh. 2 - Prob. 14CTCh. 2 - A textbook states that only five nucleotide bases...Ch. 2 - Using the following terms, fill in the following...
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